珍稀濒危植物毛柄小勾儿茶的离体培养

Tissue Cultures of Berchemiella wilsonii var.pubipetiolata

摘要: 以珍稀濒危植物毛柄小勾儿茶(Berchemiella wilsonii(Schneid.)Nakai var.pubipetiolata H.Qian)嫩梢为外植体进行组织培养实验,结果表明:以1/2 MS+6-BA 0.5 mg/L+NAA 0.05 mg/L为芽诱导培养基、MS+6-BA0.5 mg/L+NAA 0.05 mg/L为增殖培养基,可以得到大量的毛柄小勾儿茶试管苗。

Abstract: The tissue culture of Berchemiella wilsonii(Schneid.) Nakai var.pubipetiolata H.Qian was studied.The stem apex was used as explants and several kinds of MS media with different hormone combinations were tested in the experiment.The result showed that the optical media for the inducement of bud was 1/2 MS+6-BA(0.5 mg/L+)NAA 0.05 mg/L,the beneficial media for seedling growth was MS+6-BA 0.5 mg/L+NAA 0.05 mg/L.