Abstract: Cytological studies on the mode of gamete formation in F₁, hybrid between E.intermedium and Yannong 15 were carried out. The results indicated that the first meiotic division of F₁, hybrid was normal, anucleate cells and cells with micronuclei whichwere produced by asymmetrical separation of chromosomes were observed at the secondmeiotic division. The chromosome numbers of microspores ranged from 24 to 35,and thechromosome numbers of female garnetes of F₁, hybrid ranged from 20 to 35 which werereckoned from BC₁, plants. The chromosome averages of root tip cells and PMCs of F₂, hybrid were 54. 78 and 56. 41, respectively. Therefore, incomplete reduced garnetes wereproduced in meiosis of F₁, PMC,which were mainly taken place at the second meiotic division.