Abstract: Alarge number of adventitious buds were induced from in vitro cultured young panicles of haploids of rice.The adventitious buds subcultured on solidified subculture media at 26℃ for 7 days were loaded into 18 mLplastic cryotubes with enough cryoprotectant and kept on ice for 45～60 min.After cooled at a rate of 1.0℃/min down to -40℃, the samples were plunged into liquid nitrogen.The adventitious buds cryopreserved for about 30 days were thawed rapidly in 38～40℃ water and then plated on solidified MSmedium containing 3% sucrose, 0.5 mg/L NAAand 2.0 mg/Lkinetin.After plated, 21%～29% of adventitious buds resumed growth and 14%～18% regenerated plantlets.The results of this work indicated that selecting adventitious buds derived from in vitro cultured young panicles is a critical factor for the success and subculturing adventitious buds on MSmedium containing 3% sucrose and 4% sorbitol or 20% potato extract is essential to the procedure.The effective cryoprotectant is 10% DMSO (dimethyl sulfoximide)+0.5 mol/Lsorbitol.Chromosome identifying of root tip cell and RAPDanalysis of regenerating plants indicated that the regenerating plants have good genetic stability.