Abstract: Young leaves of open pollinated strawberry (Fragaria×ananassa Duch) seedlings developed adventitious shoots when they were cultured in vitro on Murashige and Skoog high mineral salts (MS) supplemented with 6-benzylaminopurine (6-BAP) (1.5 to 2.Stag/liter) and 3-naphthaleneacetic acid (NAA) (0.1 mg/liter). About 20% of all explants formed shoots. Once shoots had formed, they proliferated readily on these media. When callus was transferred to these media, no shoots formed. The substitution of indole-3 acetic acid for NAA gave media that produced no shoots from cither leaves or callus.