高级检索+

野生稻的遗传学研究——Ⅱ、普通野生稻过氧化物酶同工酶的多态性研究

GENETIC STUDIES ON THE WILD RICE (ORYZA)——Ⅱ、The polymorphism of peroxidase isozymes of common wild rice

  • 摘要: 本文利用电泳方法,研究了普通野生稻,尤其是中国和印度普通野生稻的过氧化物酶同工酶的多态性,结果表明: 1).普通野生稻中,过氧化物酶同工酶能分离出24条酶带,各酶带的出现频率不同,其中第3、5、6、9、10、11、19、20、21和22号酶带出现频率超过50%,又以第3、20和22三条酶带出现频率最高,这10条带可以做为普通野生稻过氧化物酶同工酶的基本酶谱,而第3、20和22三条带可能是其原始酶带。2).中国和印度的普通野生稻过氧化物酶同工酶有一定差异,但在墓本酶谱和原始酶带的表现上是一致的。因此,尽管普通野生稻过氧化物酶同工酶具有高度的分子多态性,但仍能反映种的分子特征。本文还讨论了过氧化物酶同工酶与普通野生稻的演化,和我国栽培稻的起源及其在进化学研究中的价值。

     

    Abstract: The polymorphism of peroxidase isozymes of the common wild rice, particularly China and India common wild rice was studied by using isozyme electrophoresis methods. The results were:1) 24 bands of peroxidase were separated in the common wild rice. The frequence of differential hand is different from each other. The frequence of the 3rd, 5th, 6th, 9th, 10th, 11th, 19th, 20th, 21th and 22th bands is equal to 50 percent and more, among which the 3rd, 20th, 22th appear the most frequently. This suggests that the 10 bands can be treated as the basic zymogram, and the 3th, 20th, 22th may be the original bands of peroxidase in the common wild rice.2) The zymograms of peroxidase in China and India common wild rice differ, however are similar to in the basic zymogram and the original band.Therefor, although the comlnon wild rice shows higher polymorphism of peroxidase, its specific molecular characters are obvious. According to the study of peroxidase isozymes, the origin of cultivated rice and the evolution of the common wild rice are discussed. The significance of peroxidase isozymes in evolutionary studies is also discussed.

     

/

返回文章
返回