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枣胚乳三倍体的细胞学——中国果树资源细胞学研究之六

CYTOLOGY OF ENDOSPERM-TRIPLOID IN ZIZYPHUS SATIVA

  • 摘要: 枣属植物经过染色体鉴定的5个种,未发现奇数倍性;中国枣为二倍体,未见自然多倍体类型。1978年通过胚乳培养,已首次诱导出三倍体植株。1983年三倍体始花结果,其细胞学特点如下: 1.胚乳二倍体胚乳二倍体的染色体数2n=24。小孢子母细胞减数分裂染色体行为正常。前期Ⅰ和中期Ⅰ,形成12个二阶体。减数分裂结束,形成正常的四分体,小孢子大小整齐。花粉粒属于正常的三孔沟型。2.胚乳三倍体胚乳三倍体的染色体数2n=36。小孢子母细胞较二倍体大。减数分裂染色体行为不正常。前期Ⅰ和中期Ⅰ有数目不等的单价体、二价体和多价体,染色体群数变动于14—20之间。后期Ⅰ、Ⅱ染色体分离不规则,数目不均衡,有落后染色体,多极分裂,并带有微核。减数分裂结束时,部分小孢子母细胞形成一分体、二分体、不等四分体、五分体和六分体等,小孢子大小不一致。正常花粉比二倍体大,有三孔沟和四孔沟两种类型,还有部分小花粉粒和败育花粉。

     

    Abstract: Chromosome numbers of 5 Zizyphus species are known. There is no odd ploidy, Zizyphus sativa Gaertn. is diploid, and no spontaneous polyploid type has been found. The triploid plants were first induced from endosperm of Z. sativa in 1978. The endosperm-triploid began to blossom. and bear fruits in 1983. Its cytological behavior is as follows:1. The endosperm-diploidChromosome number of diploid is 2n = 24. Meiotic behavior is regular. At prophase Ⅰ and metaphase Ⅰ, 12 bivalents were observed. At the end of meiosis, normal tetrads occur, and the microspores are equal in size. The pollen grains are normal tricolporate ones.2. The endosperm-triploidChromosome number of triploid is 2n = 36. Its microsporocytes are larger than diploid. Meiotic behavior is very irregular. At prophase Ⅰ and metaphase Ⅰ, there are variable numbers of univalents, bivalents and multivalents, with the numbers of chromosome groups varying from 14 to 20. At anaphase Ⅰ, Ⅱ, disjunction is irregular, resulting in unequal chromosome numbers, lagging chromosomes, multipolar division and micronuclei. At the end of meiosis, some microsporocytes give rise to monads, diads, unequal tetrads, pentads and ha xads, and the microspores are not uniform in size. The normal pollen grains are larger than diploid, and are tricolporate or tetracolporate. Some small and aborted pollen grains were found.

     

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