Abstract: The present paper reports the karyotype of Fritillaria huangshanensis Y. K. Yang et C. J. Wu and F. monantha Migo. The former was collected from Jiaugxi, its karyotype fomula in 2ⁿ=24=2^m+2^sm+6^st(4^sc)+14^t. The latter was collected from Zhejiang, its karyotype formula is 2ⁿ=24=2^m+2^sm+6^st(4^sc)+14^t(2^sc). The karyotypes of two species were studied for the first time.F. huangshanensis, F. monantha, F. thunbergii, F. anhuiensis and F. hupehensis native to middle and lower reaches of the Changjiang River, have highly similar karyotypical characteristic. The 5 species comparing with F. olgae, F. verticillata, F. yuminensis, F. walujewii and F. pallidiflora native to Xinjiang Northwest China, the karyotype asymmetrical degree of them has increased, the number of secondary constrictions has decreased (from 8—16 reduce to 2—6 ), and the secondary constrictions is only found on the long arms. According to the karyotype analysis, they are a group of evolutionarier population than that of from Xinjiang.