Abstract: The comparetive studies of esterase and α-amylase isozymes on the bamhoo rhizome systems with different developmental ages, and standing culms in same rhizome with a series of growthages were made by means of polyacrylamide gel electrophoresis. The samples were from edible bamboo species for giant pandas in sub-mountain areas, including seedlings, full-grown and flowering bamboo plants. The results showed that the leaf EST can present various electrophoretic patterns for different rhizome systems of the same species. But the Shifts in AMY isozymes appear related to age level of bamboo culms. As a consequence it is suggested that two of above mentioned isozyme systems may be used as a physiological index for development analysis of bamboo clone.