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不同胁迫处理对刺栲叶片叶绿素a荧光的影响

Effects of Different Stress Treatments on Chlorophyll a Fluorescence in Detached Leaves of Castanopsis hystrix

  • 摘要: 探讨了光强、温度和水分胁迫对刺栲(Castanopsis hystrix)体叶片叶绿素a荧光特征的影响,由此了解它的基本生物学特性。结果表明:(1)强光(1 300μmol·m-2·s-1左右)未胁迫时下降6.1%,表现轻微光抑制;(2)黑暗下低温(4℃,72 h)处理后电子传递速率下降较少(21.1%),初始荧光Fo保持稳定;与25℃对照相比,Fv/FoFv/Fm无显著变化,表明黑暗下零上低温对PSⅡ潜在活性及光化学效率影响较小;(3)高温(40℃)胁迫显著影响了PSⅡ反应中心活性,ETR,Fv/FmFv/Fo下降在处理2 h后达到极显著水平(p<0.01),Fo上升在处理4 h后也达到显著水平(p<0.05);(4)PEG诱导的水分胁迫严重影响了其光合机构活性,表现在无论与处理前比较、还是与耐旱种降真香(Acronychia pedunculata)的比较,其Fo上升和Fv/FmFv/Fo下降都达到显著水平,表明其不耐干旱。

     

    Abstract: In order to learn about some basic biological characteristics of Castanopsis hystrix,one dominant or constructive tree species in the subtropical evergreen broad-leaved forests,effects of radiation,temperature and water stress on several parameters of chlorophyll a fluorescence in detached leaves of(C.hystrix)were studied.The results showed that:(1)The Fv/Fm(maximal photochemical efficiency of PSⅡ)in leaves of C.hystrix only decreased 6.1% after the treatment by strong light intensity,comparing to Fv/Fm in leaves of Psychotria rubra,a shade-tolerant shrub,which had a stronger inhibition((17.17%))under same light stress,suggesting C.hystrix had favourable adaptation to sun environment;(2)Little decrease in ETR(electron transport rate)with consistent Fo in the detached leaves treated by 4℃ for 72 h indicated the insensitivity to short-time chilling;(3)No significant difference in Fv/Fo and Fv/Fm demonstrated little influence on PSⅡ potential activity and maximal photochemical efficiency of(PSⅡ)by chilling treatment in the dark;(4)A significant decrease in ETR,Fv/Fm and Fv/Fo((p<0.01))and the great increase in Fo(p<0.05)by hot shock at 40℃ to the leaves for 2 h and 4 h,respectively,showed potential damage to its photochemical reaction center;(5)Increase in Fo by(44.7%)and decrease in Fv/Fm by 22.4% and Fv/Fo by 54.4% in the leaves with water detect simulated by PEG after one-day treatment independently reflected inhibition of the potential efficiency suggesting(intolerance)to drought for C.hystrix.

     

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