木豆凝集素对蚜虫的抗性研究
Anti-aphid Effect of Pigeonpea Lectin
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摘要: 用盐析法提取10个木豆品种的凝集素,经亲和层析法提纯后添加到全纯人工营养液用于桃蚜饲养,发现所有供试木豆的凝集素对桃蚜都具有不同程度的抗拒性,抗性强弱顺序为:ICP 8863>ICPL 85010>ICPL 99066>ICPL 7035>ICPH 8>ICPL 9008>ICP 6997>“海南种”>ICPL 87091>ICPL 87119。Abstract: Pigeonpea lectin was extracted by ammonium sulfate fractionation.After purified by affinity chromatography on a ConA-Sepharose 4B column,it was added into an artificial holidic diet to feed aphids.The result showed that lectin from all the varieties of pigeonpea had anti-aphid effect to various extensions,which may be sorted descending as ICP 8863>ICPL 85010>ICPL 99066>ICPL 7035>ICPH 8>ICPL 9008>ICP 6997 >HaiNan>ICPL 87091>ICPL 87119.