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外源亚精胺对不同盐浓度下黄瓜幼苗可溶性蛋白表达的影响

Effects of Exogenous Spermdine on Soluble Protein Expression in Cucumber Seedlings under Different Saline Concentrations

    摘要
  • 摘要: 采用营养液水培的方法,以黄瓜品种‘津春2号’为试材,研究了叶片喷施外源亚精胺(Spd)对不同浓度NaCl(0、50、75、100 mmol·L-1)胁迫下幼苗植株可溶性蛋白表达的影响。结果表明,50 mmol·L-1NaCl胁迫下植株叶片和根系中可溶性蛋白含量提高;75 mmol·L-1和100 mmol·L-1NaCl胁迫明显降低了幼苗植株叶片和根系中可溶性蛋白的含量。不同浓度NaCl胁迫下喷施Spd后可溶性蛋白含量在根系中没有明显变化规律,而叶片可溶性蛋白含量提高。SDS-聚丙烯酰胺凝胶电泳(SDS-PAGE)对黄瓜幼苗根系可溶性蛋白分析表明,至少有3种分子量约为61.5、50、47 kD蛋白在50 mmol·L-1和75 mmol·L-1NaCl胁迫下表达量增强,但100 mmol·L-1NaCl胁迫下变化不明显;50 mmol·L-1和75 mmol·L-1NaCl胁迫下喷施Spd后61.5 kD和47 kD蛋白表达量明显减弱,50 kD蛋白甚至消失,100 mmol·L-1NaCl胁迫下61.5 kD和47 kD蛋白无明显变化,50 kD蛋白反而增强。

     

    Abstract: Effects of exogenous spermdine application on soluble protein expression in seedlings of cv.‘Jinchun No.2’ cucumber(Cucumis sativus L.) were conducted under different salinity treatments by hydroponic culture.The results showed that the soluble protein content both leaves and roots significantly increased under 50 mmol·L-1,but decreased under 75 mmol·L-1 and 100 mmol·L-1 salt treatments.Exogenous 1 mmol·L-1 spermdine spray applied increased the soluble protein content in leaves,but no significant changes in roots.By SDSPAGE electrophoresis analysis,61.5 kD,50 kD and 47 kD protein bands were increasing expressed in roots under 50 mmol·L-1 and 75 mmol·L-1 salt concentration,but not obviously expressed under 100 mmol·L-1 salt concentration.However,when 1 mmol·L-1 spermdine was applied,61.5 kD and 47 kD protein bands slimly expressed,interestingly,50 kD protein band disappeared even under 50 mmol·L-1 and 75 mmol·L-1 salt treatments,but increasing salt concentration to 100 mmol·L-1,61.5 kD and 47 kD protein bands had not obvious changes,then 50 kD protein band expressed obviously.

     

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