Abstract: The composition and content of glucosinolates in root,stem and leaf of rapeseed(Brassica napus) were analyzed.The results shows that within root 4-methoxyglucobrassicin and neoglucobrassicin predominated,while in leaf and root tissue progoitrin and gluconasturtin were the most abundant.From pre-winter stage to final flowering stage,the profile of glucosinolates in root were constant,while the contents of glucosinolates were congruously variable,highest in final flowering stage,and lowest in mature stage.There were significant differences between the profile and concentration of glucosinolates in root of 19 rape varieties,which may partially account for differences in inhibition of B.cinerea mycelium growth by hydrolytic compounds release by root of different rape varieties.It can be presumed that B.cinerea is very susceptible to butenyl ITC,and the mycelium growth inhibition of indole ITC was less than that of(aromotic) ITC.