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胚乳自主发生型龙须草FIE基因部分cDNA序列克隆和分析

Cloning and Analyzing of Partial cDNA Sequences of FIE in Autonomous Endosperm Type Eulaliopsis binata

  • 摘要: 根据拟南芥(Arabidopsis thaliana)、水稻(Oryza sativa)、玉米(Zea mays)等物种的FIE序列的保守区域设计简并引物,以龙须草(Eulaliopsis binata)的花序为材料,抽提RNA,用RT-PCR的方法扩增到800 bp左右的片断,将其克隆到pGEM-T载体上并测序。结果表明该片断与已报道的玉米、高粱(Sorghum halepense)和水稻等FIE同源基因具有较高的相似性,为龙须草FIE基因特异片断。

     

    Abstract: RNA was extracted from inflorescence of Eulaliopsis binata and used as template for cloning by RT-PCR with degenerate primers which were designed according to the FIE homologous sequences of Arabidopsis,rice and maize.As a result,one about 800 bp fragment was obtained and then cloned and sequenced.Analysis with partial cDNA sequence and deduced amino acid sequence suggested that the fragment was similar to the reported FIE homologous genes of maize,sorghum and rice.The partial cDNA is a part of FIE gene in Eulaliopsis binata.

     

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