Abstract: The effect of different spore storage time and cultivate conditions on the formation of gametophyte and sporophyte in Adiantum reniforme var.sinense was investigated in present study.The results showed that the spores of A.reniforme var.sinense was non-chlorophyllous spores and could remain viable in ambient storage for at least five years,but different storage time has a significant effect on formation time of both gametophytes and sporophytes,with that 7 days ambient storage resulting gametophyte formation in 29 days prolonged five year ambient storage resulting sporophyte formation in 38 days,whereas 7 days ambient storage resulting sporophyte formation in 96 days prolonged four month storage resulting sporophyte formation in 105 days.The culture condition(controlled temperature and light:(25±1)℃ and 2000-2500 lx for 12 h/day)in sand media resulted in a shortest sporophyte formation time,87 days after sporaphyte was inoculated.The sand medium is more favorable than peat soil for sporophyte formation and high density of gametophytes enhanced the sporsphyte formation.