Analysis of Electrophoretic Pattern of Seed Protein of Four Cultivated Species in Cucurbita
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摘要: 通过聚丙烯酰胺凝胶电泳(PAGE)对南瓜属4个栽培种中国南瓜、印度南瓜、美洲南瓜及黑籽南瓜的12个品种种子蛋白组分进行了分析。结果显示,黑籽南瓜种子以盐溶蛋白条带居多;其它3个栽培种以水溶蛋白条带居多,其次为盐溶蛋白;4个栽培种酸溶蛋白条带均较少;醇溶蛋白未分离得到蛋白谱带。水溶、盐溶、酸溶蛋白电泳图谱显示各栽培种间蛋白谱带差异明显,同一栽培种品种间蛋白谱带较为一致。
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关键词:
- 南瓜属 /
- 种子蛋白 /
- 聚丙烯酰胺凝胶电泳(PAGE)
Abstract: Using polyacrylamide gel electrophoresis(PAGE)to anlysis seed protein ingredients of 12 cultivars from four cultivated species in genus Cucurbita:C.moschato Duch,C.maxima Duch,C.pepo L.,C.ficifolia.The results showed:Among seed proteins extracted with water,0.5 mol·L-1 NaCl,0.1 mol·L-1 Lacetic acid and 70% Ethanol,most of them are salt-soluble proteins in C.ficifolia;In the other three cultivated species,most of them are water-soluble proteins,with little salt-soluble proteins;All of the four species have very little acid-soluble proteins;In the examination,no alcohol-soluble protein bandings were obtained.The maps of water-soluble proteins,salt-soluble proteins,acid-soluble proteins showed great difference among the four species,but there were almost no difference among the cultivars of the same specie.-
Keywords:
- Cucurbita /
- Seed proteins /
- Polyacrylamide gel electrophoresis(PAGE)
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