Abstract: RAPD technique has been used to construct DNA fingerprints and analyze genetic diversity in Flos lonicerae.From eighty primers,twenty-five primers were selected which can amplify clear and reproducible polymorphic bands.The PCR results showed that a total of 170 bands were amplified,of which 153 bands were polymorphic.Interestingly,primer SBSD06 generated distinct fingerprinting patterns that could distinguish each of the five lines.Genetic similarity correlation and the cluster analysis indicated the abundant genetic diversity in five F.lonicerae lines.A dendrogram was constructed based on genetic distances using UPGMA method.The dendogram reflected that five F.lonicerae lines clustered into two groups,and this was in accordance with the result of morphologic taxa.