灰绿藜幼嫩花序的组织培养及植株再生

Tissue Culture and Plant Regeneration from Immature Inflorescence Explants of Chenopodium glaucum L.

摘要: 选用盐碱地灰绿藜(Chenopodium glaucum L.)幼嫩花序为外植体,建立了快速而高效的离体组织培养体系。在附加1.0 mg/L 6-BA和0.4 mg/L IBA的MS培养基上培养35 d可诱导出不定芽,诱导频率达到66.7%;不定芽在此培养基上可快速扩增和长期继代培养。不定芽转至1/2 MS+NAA 0.2 mg/L培养基中培养2-3周,生根形成完整植株。

Abstract: A protocol was reported for high frequency adventitious shoot induction and plantlet regeneration from immature inflorescence explants of Chenopodium glaucum.Adventitious shoots were induced from immature inflorescence cultured on MS medium supplemented with 1.0 mg·L^-1 6-BA and 0.4 mg·L^-1 IBA for 35 days.Induction frequency of adventitious shoots was about 66.7%.Adventitious shoots were further multiplied vigorously and maintained for a long time on the same medium for adventitious shoot induction.Rooting was achieved after adventitious shoot was transplanted to 1/2 MS+NAA 0.2 mg/L medium for 2-3 weeks.