中国莲(<em>Nelumbo nucifera</em>)幼苗抗氧化系统对砷胁迫的响应

樊香绒; 尹黎燕; 李伟; 常锋毅

doi:10.3724/SP.J.1142.2013.60570

中国莲(Nelumbo nucifera)幼苗抗氧化系统对砷胁迫的响应

樊香绒^1,2,3,
尹黎燕^1,2,
李伟^1,2, ,,
常锋毅^1,2, ,

1.
中国科学院水生植物与流域生态重点实验室, 武汉 430074
2. 中国科学院武汉植物园水生生物学实验室, 武汉 430074
3. 中国科学院大学, 北京 100049

基金项目: 武汉市学科带头人计划项目（长江中下游浅水湖泊稳态转换及其调控机制的实验生态学研究）；中国科学院水生生物研究所淡水生态与生物技术国家重点实验室开放基金（2012FB21）。

详细信息

作者简介:
樊香绒（1988- ），女，硕士研究生，主要从事生态毒理学研究（E-mail：bestxiangrong@163.com）。

通讯作者:
李伟, E-mail: liwei@wbgcas.cn

常锋毅, E-mail: changfy@wbgcas.cn

中图分类号: Q945.78
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出版历程
- 收稿日期: 2013-05-23
- 修回日期: 2013-10-06
- 发布日期: 2013-12-29

Responses of the Antioxidant System in Nelumbo nucifera Seedlings to Arsenic Toxicity

FAN Xiang-Rong^1,2,3,
YIN Li-Yan^1,2,
LI Wei^1,2, ,,
CHANG Feng-Yi^1,2, ,

1.
Key Laboratory of Aquatic Botany and Watershed Ecology, Chinese Academy of Sciences, Wuhan 430074, China
2. Laboratory of Aquatic Plant Biology, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, China
3. University of Chinese Academy of Sciences, Beijing 100049, China

摘要

摘要: 为了研究中国莲（Nelumbo nucifera）抗氧化系统对砷胁迫的响应，研究比较了两种不同价态无机砷As（Ⅲ）和砷As（Ⅴ）对中国莲幼苗可溶性蛋白含量、丙二醛（MDA）含量以及抗氧化系统的影响。结果表明，中国莲幼苗中MDA和可溶性蛋白质含量随砷浓度的增加呈现先升高后降低的趋势。中国莲幼苗的MDA和蛋白质含量受As（Ⅴ）的影响不如As（Ⅲ）敏感。抗氧化系统酶中，超氧化物歧化酶（SOD）对砷处理最敏感，当As（Ⅲ）浓度在2.5 μmol/L和As（Ⅴ）浓度在100 μmol/L时，SOD酶活性显著高于对照组。过氧化物酶（POD）在As（Ⅲ）处理浓度为10 μmol/L时就出现显著上升，相对而言，过氧化氢酶（CAT）对As（Ⅴ）比较敏感。实验结果表明，随着浓度的增加，砷对幼苗产生的氧化胁迫导致SOD、CAT 和POD 三种酶活性有所增加，以配合清除细胞内的活性氧自由基（ROS），维持细胞代谢的稳定。本研究为进一步研究砷胁迫下莲的生理和生长变化、以及莲的培育和移植提供了部分基础数据。
- 中国莲 /
- 抗氧化系统 /
- 砷
Abstract: To study the antioxidant system of Nelumbo nucifera seedlings in response to arsenic stress, compared the influence of two valences of inorganic arsenic, As (Ⅲ) and As (V), on soluble protein content, malondialdehyde(MDA)and the antioxidant system. Results showed that: MDA and soluble protein content of N. nucifera seedlings increased and then decreased with increasing concentration; MDA and protein content were more sensitive to As(Ⅲ)than to As(V)treatment. The Superoxide Dismutase(SOD)was the most sensitive to arsenic treatment; when the concentration of As (Ⅲ)was 2.5 μmol/L and As(V)was 100 μmol/L, the activity of SOD increased significantly. When the concentration of As(Ⅲ) was 10 μmol/L, the activity of Peroxidase(POD)increased significantly. The Catalase(CAT)was more sensitive to As(V). From the experimental results, with the increase in arsenic concentration, the enzyme activities of SOD, CAT and POD increased, which can be used to eliminate reactive oxygen species(ROS) and maintain cell metabolism stability. This study provides some basic data for further study of N. nucifera physiology and growth change, cultivation and transplants with arsenic stress.
- Nelumbo nucifera /
- Antioxidant systems /
- Arsenic

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参考文献(20)

[1]	洪雪花,张婉静,张爱,刘贵明,杨良,李作生.环境砷的存在状态、生物转化和修复研究进展[J].云南农业大学学报, 2011, 26(4):567-571.
[2]	Shri M,Kumar S,Chakrabarty D,et al.Effect of arsenic on growth,oxidative stress,and antioxidant system in rice seedlings [J].Ecotoxicol Environ Saf, 2009, 72:1102-1110.
[3]	LI C X.Effects of arsenic on seed germination and physiological activities of wheat seedlings [J].J Environ Sci, 2007, 19:725-732.
[4]	Rai S,Wahile A,Mukherjee k.Antioxidant activity of Nelumbo nucifera(sacred lotus) seeds [J].J Ethnopharmacol, 2006, 104:322-327.
[5]	Bradford A,Handy R D,Readman J W,et al.Impact of silver nanoparticle contamination on the genetic diversity of natural bacterial assemblages in estuarine sediments [J].Environ Sci Technol, 2009, 43(12):4530-4536.
[6]	李合生.植物生理生化实验原理与技术:面向 21世纪课程教材[M].北京:高等教育出版社, 2000.
[7]	Cakmak I,Marschner H.Magnesium-deficiency and high light-intensity enhance activities of superoxide-dismutase,ascorbate peroxidase,and glutathione-reductase in bean-leaves [J].Plant Physiol, 1992, 98(4):1222-1227.
[8]	Giannopolitis C N,Ries S K.Superoxide dismutases.1.Occurrence in higher-plants [J].Plant Physiol, 1977, 59(2):309-314.
[9]	Xing W,Huang W,Liu G.Effect of excess iron and copper on physiology of aquatic plant Spirodela polyrrhiza (L.) Schleid [J]. Environ Toxicol, 2010, 25(2):103-112.
[10]	张继飞,陈青云,吴名.重金属镉对粗梗水蕨生长及叶片中蛋白质含量的影响 [J].江汉大学学报:自然科学版, 2011, 39(4):92-104.
[11]	Wang H F,Zhong X H,Shi W Y,et al.Study of malondialdehyde (MDA) content,superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities in chickens infected with avian infectious bronchitis virus [J].Af J Biotechno, 2011, 10(45):9213-9217.
[12]	张义贤,张丽萍.重金属对大麦幼苗膜脂过氧化及脯氨酸和可溶性糖含量的影响 [J].农业环境科学学报, 2006, 25(4):857-860.
[13]	Blokhina O,Fagerstedt K V.Reactive oxygen species and nitric oxide in plant mitochondria:origin and redundant regulatory systems [J]. Physiol Plant, 2010, 138(4):447-462.
[14]	Jaspers P,Kangasjarvi J.Reactive oxygen species in abiotic stress signaling [J].Physiol Plant, 2010, 138(4):405-413.
[15]	Swanson S,Gilroy S.ROS in plant development [J].Physiol Plant, 2010, 138(4):384-392.
[16]	Verma S,Dubey R S.Lead toxicity induces lipid peroxidation and alters the activities of antioxidant enzymes in growing rice plants [J].Plant Sci, 2003, 164(4):645-655.
[17]	Xing W,Li D,Liu G.Antioxidative responses of Elodea nuttallii (Planch.) H.St.John to short-term iron exposure [J].Plant Physiol Biochem, 2010, 48(10-11):873-478.
[18]	Sinha S,Saxena R.Effect of iron on lipid peroxidation,and enzymatic and non-enzymatic antioxidants and bacoside-A content in medicinal plant Bacopa monnieri L.[J].Chemosphere, 2006, 62(8):1340-1350.
[19]	黄辉,李升,郭娇丽.镉胁迫对玉米幼苗抗氧化系统及光合作用的影响 [J].农业环境科学学报, 2010,29(2):211-215.
[20]	于方明,刘可慧,刘华.镉污染对水稻不同生育期抗氧化系统的影响 [J].生态环境学报, 2012,21 (1):88-93.