Abstract: Spatial metabolomics is an emerging research technology that integrates mass spectrometry imaging and metabolomics to analyze the structure, concentration, and spatial distribution of endogenous metabolites within biological tissues. This approach enables the acquisition of both known and unknown metabolite information at high spatial resolution, allowing for precise localization within tissues. It is crucial for elucidating the synthesis, accumulation, and regulation of plant metabolites. This article reviews the current research status of spatial metabolomics technology, with a focus on cutting-edge applications in plant tissue research. Special attention is given to its potential and challenges in the field of single-cell plant studies, aiming to provide new avenues for studying plant growth and development and regulating spatial metabolic networks. Additionally, this technology offers crucial insights for solving problems in agricultural production, plant-based energy development, and other fields.