龙牙百合3个地方品种的形态特征及核型分析

杨利平; 符勇耀; 范军好; 黄渝

doi:10.11913/PSJ.2095-0837.2019.50559

龙牙百合3个地方品种的形态特征及核型分析

长江师范学院现代农业与生物工程学院, 重庆涪陵 408100

基金项目:

国家自然科学基金青年项目（31500245）；教育部春晖计划项目（Z2016182）；重庆市科技局基础研究与前沿探索项目（cstc2019jcyj-msxmX0014）；重庆市教委科学研究项目（KJQN201801428）；重庆市人力社保局2017年出站留（来）渝博士后科研资助项目（0108/01096101）；长江师范学院引进人才科研资助项目（2017KYQD63）；长江师范学院生物工程与现代农业专业群科研资助项目（CSZKY1813）。

详细信息

作者简介:
杨利平(1962-),男,博士,教授,研究方向为花卉栽培与种质资源创新(E-mail:836711655@qq.com)。

通讯作者:
符勇耀,E-mail:34331107@qq.com

中图分类号: S644.1
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出版历程
- 收稿日期: 2019-01-03
- 网络出版日期: 2022-10-31
- 发布日期: 2019-10-27

Morphological characteristics and karyotype analysis of three local varieties of Lilium brownii var. viridulum

School of Advanced Agriculture and Bioengineering, Yangtze Normal University, Chongqing 408100, China

Funds:

This work was supported by grants from the National Natural Science Foundation of China (31500245), Ministry of Education, "Chunhui Plan" (Z2016182), Basic Research and Frontier Exploration of Chongqing Science and Technology Bureau (cstc2019jcyj-msxmX0014), Youth Science and Technology Project from Chongqing Education Science Committee (KJQN201801428), Postdoctoral Research Funding Support from Human Resources and Social Security Bureau of Chongqing (0108/01096101), Research Funding Project of Yangtze Normal University (2017KYQD63), and Research Funding Project of Bioengineering and Modern Agriculture Specialty Group of Yangtze Normal University (CSZKY1813).

摘要

摘要: 采用经典测量和染色体常规压片法，对龙牙百合（Lilium brownii var.viridulum Baker）3个地方品种的形态特征及核型进行研究。植株形态分析结果显示：‘江西’龙牙的株高、开花口径、种球重量和周长、中外层鳞片重量和长度以及鳞片扦插产生小鳞茎数等指标均显著大于‘大叶’龙牙和‘平头’龙牙；‘大叶’龙牙的叶片最长，均值为14.54 cm。花粉、叶表皮气孔及鳞片淀粉粒的微形态特征分析结果显示：‘江西’龙牙的花粉粒径最大，均值达111.76 μm；‘平头’龙牙的叶表皮气孔最长，气孔密度也最大（约47.6个/mm²）；‘大叶’龙牙的淀粉粒径最大，均值为47.61 μm；‘江西’龙牙的淀粉粒大小分布更集中，差异性小。染色体核型分析结果显示：龙牙百合3个品种的染色体数目均为2n=2x=24，为二倍体，其中‘江西’龙牙核型公式为2n=2x=24=2m（2SAT）+6sm（2SAT）+12st（4SAT）+4t；‘平头’龙牙核型公式为2n=2x=24=4m+8sm+10st（4SAT）+2t；‘大叶’龙牙核型公式为2n=2x=24=2m（2SAT）+6sm+14st（4SAT）+2t，三者核型均为3B型。
- 龙牙百合 /
- 地方品种 /
- 形态特征 /
- 核型分析 /
- 品种鉴定
Abstract: We investigated the morphological characteristics and chromosome karyotypes of three local varieties of Lilium brownii var. viridulum (‘Jiangxi’, ‘Pingtou’, and ‘Daye’ lilies) by classical measurements and chromosome routine tableting. Plant morphological analysis indicated that the plant height, flowering diameter, bulb weight and size, scale weight and size in the outer and/or middle layer of bulb, and germination number of scales in ‘Jiangxi’ were significantly larger than those in ‘Daye’ and ‘Pingtou’; furthermore, the leaves of ‘Daye’ were the longest, with a mean value of 14.54 cm. Micromorphological feature analysis revealed that the pollen grains in ‘Jiangxi’ were the largest, with a mean size of 111.76 μm. The leaf epidermis in ‘Pingtou’ showed the longest stomata and the maximum stomata density, with a mean value of 47.6 individual/mm². In addition, the starch grains in ‘Daye’ were the largest, with an average particle size of 47.61 μm, and the starch grain size distribution in ‘Jiangxi’ was more consistent than that in others. Karyotype analysis showed that the chromosome numbers of the three local varieties were diploid, with the formula 2n=2x=24. The karyotype formula of ‘Jiangxi’ was 2n=2x=24=2m(2SAT) + 6sm(2SAT) + 12st(4SAT) + 4t, of ‘Pingtou’ was 2n=2x=24=4m + 8sm + 10st(4SAT) + 2t, and of ‘Daye’ was 2n=2x=24=2m(2SAT) + 6sm+14st(4SAT) + 2t. All three karyotypes belonged to type 3B.
- Lilium brownii var. viridulum /
- Local variety /
- Morphological feature /
- Karyotype analysis /
- Variety identification

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