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用于通路(Gateway)克隆技术的植物表达载体研究进展

肖素勤, 孙振, 轩秀霞, 陈丽梅

肖素勤, 孙振, 轩秀霞, 陈丽梅. 用于通路(Gateway)克隆技术的植物表达载体研究进展[J]. 植物科学学报, 2012, 30(5): 528-544. DOI: 10.3724/SP.J.1142.2012.50528
引用本文: 肖素勤, 孙振, 轩秀霞, 陈丽梅. 用于通路(Gateway)克隆技术的植物表达载体研究进展[J]. 植物科学学报, 2012, 30(5): 528-544. DOI: 10.3724/SP.J.1142.2012.50528
XIAO Su-Qin, SUN Zhen, XUAN Xiu-Xia, CHEN Li-Mei. Research Progress on Plant Expression Vectors for Gateway Cloning Technology[J]. Plant Science Journal, 2012, 30(5): 528-544. DOI: 10.3724/SP.J.1142.2012.50528
Citation: XIAO Su-Qin, SUN Zhen, XUAN Xiu-Xia, CHEN Li-Mei. Research Progress on Plant Expression Vectors for Gateway Cloning Technology[J]. Plant Science Journal, 2012, 30(5): 528-544. DOI: 10.3724/SP.J.1142.2012.50528
肖素勤, 孙振, 轩秀霞, 陈丽梅. 用于通路(Gateway)克隆技术的植物表达载体研究进展[J]. 植物科学学报, 2012, 30(5): 528-544. CSTR: 32231.14.SP.J.1142.2012.50528
引用本文: 肖素勤, 孙振, 轩秀霞, 陈丽梅. 用于通路(Gateway)克隆技术的植物表达载体研究进展[J]. 植物科学学报, 2012, 30(5): 528-544. CSTR: 32231.14.SP.J.1142.2012.50528
XIAO Su-Qin, SUN Zhen, XUAN Xiu-Xia, CHEN Li-Mei. Research Progress on Plant Expression Vectors for Gateway Cloning Technology[J]. Plant Science Journal, 2012, 30(5): 528-544. CSTR: 32231.14.SP.J.1142.2012.50528
Citation: XIAO Su-Qin, SUN Zhen, XUAN Xiu-Xia, CHEN Li-Mei. Research Progress on Plant Expression Vectors for Gateway Cloning Technology[J]. Plant Science Journal, 2012, 30(5): 528-544. CSTR: 32231.14.SP.J.1142.2012.50528

用于通路(Gateway)克隆技术的植物表达载体研究进展

基金项目: 国家自然科学基金资助项目(30670163); 云南省中青年学术与技术带头人培养费资助项目(2004PY01-5)。
详细信息
    通讯作者:

    陈丽梅, E-mail: chenlimeikm@126.com

  • 中图分类号: Q943.2

Research Progress on Plant Expression Vectors for Gateway Cloning Technology

  • 摘要: 通路(Gateway)克隆技术是根据λ噬菌体基因组和大肠杆菌基因组之间的位点专一性重组分子机制开发的一套分子克隆新技术。利用该技术LR反应构建目的基因的表达载体时不需要经过酶切和连接等繁琐而又费时的过程,因此,可以节省很多时间。为了扩大Gateway技术在植物基因工程领域的应用,最近有很多研究机构和研究小组开发了能用于组成型或诱导型表达目的基因、基因沉默、启动子分析、蛋白质亚细胞定位、蛋白质/蛋白质相互作用、多个DNA片段的模块化组装和DNA组片段功能验证等研究用的植物表达载体。该文对这些技术的研究进展进行了综述。
    Abstract: Gateway molecular cloning technology was developed by Invitrogen based on the mechanism of site-specific recombination between λ phage DNA and the Escherichia coli genome.Construction of an expression vector for a target gene via Gateway LR reaction does not need to use restriction enzymes and a ligase,and thus is time efficient. Recently,to expand the application of Gateway technology in plant genetic engineering,several research groups have constructed Gateway destination vectors for studies on constitutive,inducible or silence expression of target genes,promoter analyses,protein localization,protein/protein interaction,DNA fragment modular assembly and functional verification of genomic fragments.Here we review research progress on the development of these destination vectors.
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出版历程
  • 收稿日期:  2012-03-01
  • 修回日期:  2012-07-10
  • 发布日期:  2012-10-29

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