Research and Evaluation on Chemical Fingerprints of Diterpenoids from Rabdosia rubescens
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摘要: 为了建立冬凌草(Rabdosia rubescens)二萜类成分的HPLC指纹图谱,并通过指纹图谱对来自不同产地和不同类型的冬凌草进行评价,本研究以10批太行山产冬凌草HPLC色谱图为参照,建立了冬凌草二萜类成分的指纹图谱,色谱条件为,色谱柱: phenomsil C18(250×4.6 mm,5 μm)柱; 流动相:甲醇∶0.5%磷酸水溶液梯度洗脱; 柱温: 30℃; 检测波长: 238 nm; 流速: 0.8 mL/min。通过化学成分指纹图谱相似度分析以及主成分分析方法,综合评价不同产地冬凌草样品质量的差异。结果表明冬凌草种质资源出现多种分化,不同地区冬凌草二萜类成分差异明显;不同变异类型冬凌草二萜类成分也发生了变化,但没有明显规律性。Abstract: In this paper,chemical fingerprints were established by referring to the HPLC chromatography of ten batch samples from the Taihang Mountains.Phenomsil C18 (250×4.6 mm,5 μm) columns were used and methanol-water (0.5% phosphoric acid.) was chosen as the mobile phase.The column temperature was 30℃ and the fingerprints were detected at 238 nm.The flow rate was 0.8 mL/min.The quality of Rabdosia rubescens herba from different places was evaluated by similarity analysis and principal component analysis of chemical fingerprints.The results showed that R.rubescens had multiple germplasm,the diterpenoids from R.rubescens in different areas had significant differences,and the diterpenoids of different mutation types were changed,but showed no obvious trend.The results of this article provide a scientific basis for the protection and development of R.rubescens germplasm.
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