Cu-NiR与cd<sub>1</sub>-NiR——两类反硝化亚硝酸还原酶研究进展

王宝茹; 王旭; 王伟波; 唐东山

doi:10.11913/PSJ.2095-0837.2021.30324

Cu-NiR与cd₁-NiR——两类反硝化亚硝酸还原酶研究进展

1. 南华大学资源环境与安全工程学院, 衡阳市土壤污染控制与修复重点实验室, 湖南衡阳 421001;
2. 中国科学院武汉植物园, 中国科学院水生植物与流域生态重点实验室, 武汉 430074

基金项目:

国家自然科学基金项目（31870452，12075113）；衡阳市土壤污染控制与修复重点实验室项目（2018HPT06）。

详细信息

作者简介:
王宝茹(1996-),女,硕士研究生,研究方向为环境生态学(E-mail:1275852921@qq.com)。

通讯作者:
唐东山,E-mail:445430009@qq.com

中图分类号: Q943.2
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出版历程
- 收稿日期: 2021-04-02
- 修回日期: 2021-04-24
- 网络出版日期: 2022-10-31
- 发布日期: 2021-06-27

Cu-NiR and cd₁-NiR: Advances in two kinds of denitrifying nitrite reductase

1. Hengyang Key Laboratory of Soil Pollution Control and Remediation, Resource Environment and Safety Engineering College, University of South China, Hengyang 421001, China;
2. Key Laboratory of Aquatic Plants and Watershed Ecology, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, China

Funds:

This work was supported by grants from the National Natural Science Foundation of China (31870452,12075113) and Hengyang Key Laboratory Project for Soil Contamination Control and Remediation (2018HPT06).

摘要

摘要: 亚硝酸还原酶（Nitrite Reductase，NiR）是自然界氮循环过程中催化亚硝酸盐还原的一类关键酶。其中，nirS和nirK基因编码合成的亚硝酸还原酶Cu-NiR和cd₁-NiR是反硝化作用中的限速酶，二者功能类似但结构和辅助因子组成截然不同。研究发现这两类酶对不同环境梯度具有不同的响应机制。因此，本文详述了Cu-NiR和cd₁-NiR的结构特征、相关微生物类群的分布和检测技术，并针对nirS和nirK基因表达和活性的影响因素进行了统计和分析，最后对未来的研究方向进行了展望。
- 亚硝酸还原酶 /
- nirK基因 /
- nirS基因 /
- 基因丰度 /
- 环境因子
Abstract: Nitrite reductase (NiR) is a key enzyme in the natural nitrogen cycle that catalyzes the reduction of nitrite. Cu-NiR and cd₁-NiR, which are encoded by the nirK and nirS genes, respectively, are rate-limiting enzymes in denitrification. Although they have similar functions, their structures and cofactors are different. In addition, they exhibit different responses to different environmental gradients. The structural characteristics, microbial population distribution, and detection techniques of these two enzymes are reviewed in this report. Furthermore, the environmental factors that determine the expression and activity of the nirS and nirK genes are examined. Finally, future research directions are proposed.
- Nitrite reductase (NiR) /
- nirK gene /
- nirS gene /
- Gene abundance /
- Environmental factors

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参考文献(53)

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