Abiotic Stress Response Mechanism of Oilseed Rape (Brassica napus L.) Revealed from Proteomics
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摘要: 油菜（Brassica napus L.）是我国的主要油料作物之一，在生长发育过程中经常受到干旱、高温、高盐和营养缺乏等非生物胁迫。这些胁迫通常会阻碍油菜的生长发育，导致品质和产量下降。近年来，快速发展的高通量蛋白质组学技术为揭示油菜胁迫响应分子机制提供了新线索。本文综合分析了油菜不同组织/器官（如：叶片、根、下胚轴和种子）在响应盐、高温、干旱、草酸和缺素（磷、硫和硼）等逆境过程中675种蛋白质的丰度变化特征，揭示了其胁迫应答机制，主要包括：（1）通过G蛋白介导的信号通路感知与传递胁迫信号；（2）通过改变参与糖类与能量代谢相关酶的丰度调节代谢水平；（3）通过叶绿素合成的变化调节光合作用；（4）调节转录因子、蛋白质合成与命运相关蛋白质的丰度，从而在转录、翻译以及翻译后修饰等水平上应答逆境；（5）通过调节膜联蛋白、V型H+-ATP酶等质膜蛋白质，促进细胞内物质吸收与转运；（6）通过细胞骨架动态重塑保持正常细胞结构；（7）利用调节抗氧化酶系统清除活性氧，并通过合成多种防御物质减轻细胞受到的伤害。本综述为解析油菜逆境应答网络体系中的关键调控及代谢通路的变化提供了重要信息。Abstract: Oilseed rape (Brassica napus L.) is one of the main oil crops in China. These plants can suffer from various abiotic stresses, such as drought, high temperature, salinity, and nutrient deficiency, which can significantly impact their growth, quality, and yield. In recent years, high-throughput proteomic investigations have provided new clues for understanding the molecular stress response mechanisms in oilseed rape. In this paper, the diverse patterns of 675 stress response proteins in different oilseed rape tissues/organs (e.g., leaves, roots, hypocotyls, and seeds) were analyzed in response to several environmental stresses (i.e.,salt, high temperature, drought, oxalic acid, and nutrient deficiency). They include:(i) perception and transmission of the stress signal by G proteins; (ii) alterations in the abundance of key enzymes involved in carbohydrate and energy metabolism; (iii) alterations in the abundance of chlorophyll synthesis enzymes to regulate photosynthesis; (iv) diverse proteins changed and interaction patterns regulated at the transcriptional, translational, and post translational levels; (v) regulating the plasma membrane proteins (e.g. annexin and V-H+-ATPase) to promote the absorption and transport of intracellular substances; (vi) remodeling of the cytoskeleton to maintain cell structure and function; (vii) detoxification of reactive oxygen species by antioxidant enzymes and antioxidants. This study provides important information for understanding the regulatory and metabolic pathways in oilseed rape in response to abiotic stresses.
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Keywords:
- Brassica napus /
- Abiotic stresses /
- Proteomics
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