Comparison of Four Different Approaches for the Extraction of Elaeagnus angustifolia Flower Volatiles
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摘要: 为了探究不同提取方法对沙枣花挥发物化学成分分析的影响,本文采用水蒸气蒸馏法、固相微萃取法、顶空采集法和气囊采集法提取沙枣(Elaeagnus angustifolia)花的挥发性物质,并利用气相色谱-质谱联用仪(GC-MS)分别对4种方法提取的挥发物质进行化学成分分析鉴定。从4种方法提取的沙枣花挥发物质中共检测出51种化合物,其中酯类14种、烷类13种、酚类9种、酮类3种、醇类4种、其它物质8种。顶空收集法、水蒸气提取法、气囊采集法和固相微萃取法提取的挥发物质中分别检出化合物13种、19种、22种和18种,并且用2种(或2种以上)方法提取的沙枣花挥发物质中相同化合物有15种(即:酯类6种、烷类5种、酚类2种、醇类1种、其它物质1种),分别为:反式-肉桂酸乙酯、顺式-肉桂酸乙酯、反式-肉桂酸甲酯、邻苯二甲酸二甲酯、正癸烷、正十二烷、正十四烷、正十六烷、正二十烷、愈创木酚、4,4'-亚甲基双(2,6-二叔丁基苯酚)、2-苯基-1-丙烯、乙酸叶醇酯、肉桂酸异丙酯和苯乙醇。4种方法提取的沙枣花挥发物中均含反式-肉桂酸乙酯和邻苯二甲酸二甲酯2种化合物,且反式-肉桂酸乙酯的含量较高,分别为83.75%(水蒸气蒸馏法)、14.45%(固相微萃取法)、78.27%(顶空收集法)和56.65%(气囊采集法)。4种方法中气囊采集法提取的沙枣花挥发物质中所含化合物种类最多(22种),而且操作最简单,因此气囊采集法是提取沙枣花挥发物的较好方法。Abstract: Elaeagnus angustifolia has a great scent, attracts many insects and is the main host of Asias halodendri. To explore the chemical compounds in E. angustifolia, four different approaches for the extraction of E. angustifolia flower volatiles were performed and compared to determine the optimum method. The chemical compounds in raw extract were identified using gas chromatography-mass spectrometry (GC-MS). A total of 51 compounds were attained among the four approaches, including 14 esters, 13 ethers, 9 phenols, 3 ketones, 4 alcohols, and 8 others. Comparison of the four approaches showed that 13 compounds were found by headspace collection (HC), 19 compounds were found by steam distillation (SD), 18 compounds were found by solid phase micro extraction (SPME) and 22 compounds were found by air bag collection (ABC). Fifteen compounds were found by two or more approaches, including ethyl cinnamate, ethyl 3-phenyl propenoatea, methyl (E)-cinnamate, dimethyl 1,2-benzenedicarboxylate,n-decane, n-dodecane, n-tetradecane, n-hexadecane,n-eicosane,2-methoxyphenol,4,4'-methylenebis(2,6-di-tert-butylphenol),2-phenyl-1-propene,leaf acetate,isopropyl cinnamate and phenethyl alcohol. Ethyl cinnamate and 1,2-benzenedicarboxylate were detected by all four measures. The ethyl cinnamate content for each material was the highest, measuring 83.75%(SD), 14.45%(SPME), 78.27%(HC), and 56.65%(ABC), respectively.
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[1] 傅德志,李德铢,包伯坚,张宪春. 中国植物志:第52卷,第2分册[M]. 北京:科学出版社, 1997: 40. [2] 马丽娟. 沙枣的开发利用[J].宁夏科技, 2000(4): 37. [3] 张凤春. 沙枣资源的利用与保护[J].自然资源, 1990(5):32-35. [4] 张万年,刘泽鹰,王哲民. 沙枣树药用价值和有效成分研究的进展[J].中草药, 1986, 17(7): 41-44. [5] 邓彦斌,刘宗渊,姜彦成,王红. 沙枣花蜜腺的发育解剖学研究[J].植物研究,1998,18(1): 34-37. [6] 廉宜君,李元元,李敏,唐娟,陈韩英,利炳奇. 超临界CO2萃取和水蒸气蒸馏法萃取沙枣花挥发油工艺的比较研究[J]. 中国中医药信息杂志, 2010,17(1): 51-53. [7] 杨继涛. 兰州沙枣花挥发性物质分析[D]. 兰州市:兰州大学,2010. [8] 乔海军,杨继涛,杨晰,赵连彪,李铁汉. 沙枣花挥发油化学成分的GC-MS分析[J].食品科学, 2011,32(16): 233-235. [9] 黄馨瑶,马晖,王小明,刘益宁. 沙枣花香气的人气调查及化学成分分析[J]. 天然产物研究与开发, 2009,21(3): 480-488,644. [10] 刘晔玮,邸多隆,王勤. 沙枣花挥发油的化学成分及其指纹图谱的研究[J]. 香精香料化妆品,2003(3): 11-13. [11] 阎鸿建,王青,韦凤英. 沙地沙枣花挥发油和精华油成分的研究[J].有机化学,1988,8(4):346-351. [12] 周民锋,孙欣阳,秦宏兵,顾海东. 固相萃取-气相色谱质谱联用测定地表水中24种半挥发性有机物[J]. 2013中国环境科学学会学术年会论文集,2013(20): 354-358. [13] Run CS, Xiao FS, Qi L. Analysis of lignins solubilized in two-stage organosolv and alkaline peroxide treatments from Haloxylon ammodendron and Elaeagnus angustifolia[J]. Int J Polym Anal Ch, 2002, 7(3):435-440.
[14] 王妍,巨涛,王立新. 超临界CO2流体萃取及气质联用研究宁夏沙枣花的芳香成分[J].香料香精化妆品,2007(3): 1-4. [15] 熊伟,金荷仙,蔡宝珍.驱蚊草挥发物化学成分分析[J].应用研究,2011,4(11): 101-121. [16] Catakin D, Gheorghe A. Supercritical carbon dio-xide extraction of Angelica archangelica L. root oil[J]. J Supercrit Fluid,1998,12(1): 58-67.
[17] 贺莉娟,梁逸曾,赵晨曦. 唇形科植物挥发油化学成分的GC/MS研究[J].化学学报,2007(3): 227-232. [18] 王妍,李玲,连松刚,王立新. 顶空-固相微萃取气质联用分析宁夏沙枣花中香气成分[J].中草药, 2007, 11(11): 1620-1627. [19] 瞿新华. 植物精油的提取与分离技术[J]. 安徽农业科学,2007,35(32):10194-10195. [20] 杨莉. 不同顶空分析法对植物挥发物测定的影响[D]. 北京:北京林业大学,2007. [21] Aviv A, Alexander B Fialkov, Tal A. What can be improved in GC-MS——When multi benefits can be transformed into a GC-MS revolution[J]. Int J Analytical Mass Spectr Chrom, 2013,6(1), 31-47.
[22] Garg GP, Khanna RN. Chemical examination of flowers of Pongamia glabra[J]. Pharm Biol, 1983, 21(1): 3-47.
[23] Vandendriessche T, Nicolai BM, Hertog MLATM. Optimization of HS SPME fast GC-MS for high-throughput analysis of strawberry aroma[J]. Food Anal Meth,2013(6): 512-520.
[24] 吴彩霞,刘红丽,卢素格,王金梅,康文艺. 固相微萃取法与水蒸气蒸馏法提取蜘蛛香挥发油成分的比较[J].中国药房,2008,19(12): 367-371. [25] Guddadarangavvanahally KJ, Kotamalli NCM, Ram MU, Bhimanagouda SP. Chemical composition of volatile oil from Citrus limettioides and their inhibition of colon cancer cell proliferation[J]. Ind Crop Prod, 2013,45:200-207.
[26] Anna M, Raspolli G, Claudia A, Monica B, Fabio P. Chitosan as biosupport for the MW-assisted synthesis of palladium catalysts and their use in the hydrogenation of ethyl cinnamate[J]. App Catal A-Gen, 2013,7:468-473.
[27] Stenhagen E, Abrabamsson S, McLafferty FW. Registry of Mass Spectral Data[M].New York: John Wiley and Sons, 1974: 1-2.
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