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HPLC-MS/MS指纹图谱模式识别结合化学计量学对文王一支笔特征标志物的差异分析

Differential analysis of feature markers of Balanophora involucrata Hook. f. by HPLC-MS/MS fingerprint pattern recognition coupled with chemometrics

  • 摘要: 文王一支笔为蛇菰科植物筒鞘蛇菰(Balanophora involucrateHook. f.)的干燥全草,是鄂西地区特色民族珍稀药材。本研究首先利用HPLC-MS/MS技术建立了鄂西十堰地区5个产地10个批次文王一支笔的根茎、花茎和花序3个部位的化学指纹图谱,标定了其中29个共有峰,并推断了27个色谱峰的化学结构,包括13个酚酸类和8个黄酮类成分。聚类分析(CA)及主成分分析(PCA)结果显示,文王一支笔地下(根茎)与地上(花茎、花序)部位化学成分差异较大,花茎与花序间化学成分差异较小。正交偏最小二乘法-判别分析(OPLS-DA)中VIP值分析结果表明,峰12、8、2、3、17、21、9和22是引起文王一支笔不同药用部位化学成分差异的潜在标志性成分(Q-marker),可作为其质量控制的依据。

     

    Abstract: Balanophora involucrateHook. f. is a rare medicinal herb with a long history of clinical use in the western Hubei region. However, comprehensive and systematic comparative analyses of its chemical components in different medicinal parts are lacking. In this study, HPLC-MS/MS technology was applied to establish the chemical fingerprints of the rhizome, flower stem, and inflorescence of 10 batches ofB. involucratefrom five production areas in western Hubei. In total, 29 common peaks were calibrated, and the chemical structures of 27 chromatographic peaks were tentatively identified, including 13 phenolic acids and eight flavonoids. Cluster analysis (CA) and principal component analysis (PCA) revealed significant differences in chemical compositions between the underground (rhizome) and aboveground(flower stem, inflorescence) parts, while differences in chemical compositions between the flower stem and inflorescence were relatively small. The variable importance in projection (VIP) values in orthogonal partial least squares discriminant analysis (OPLS-DA) indicated that peaks 12, 8, 2, 3, 17, 21, 9, and 22 were potential quality markers (Q-markers) among different medicinal parts and could serve as the chemical basis for quality control.

     

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