莲花色性状QTL定位及分子标记开发

李静; 刘正位; 朱红莲; 匡晶; 彭静; 王直新; 季群; 柯卫东

doi:10.11913/PSJ.2095-0837.2022.60820

莲花色性状QTL定位及分子标记开发

武汉市农业科学院, 武汉 430070

基金项目:

国家特色蔬菜产业技术体系项目(CARS-24-A-12)。

详细信息

作者简介:
李静(1994-)，女，硕士，研究实习员，研究方向为蔬菜分子生物学(E-mail: Lijing9406@163.com)。

通讯作者:
柯卫东,E-mail: wdke63@163.com

中图分类号: Q75
计量
- 文章访问数: 110
- HTML全文浏览量: 2
- PDF下载量: 22
出版历程
- 收稿日期: 2022-06-11
- 修回日期: 2022-08-02
- 网络出版日期: 2023-01-12

Mapping of quantitative trait loci and development of closely linked markers of flower color in lotus (Nelumbo nucifera Gaertner)

Wuhan Academy of Agricultural Sciences, Wuhan 430070, China

Funds:

This work was supported by a grant from the Earmarked Fund for CARS (CARS-24-A-12).

摘要

摘要: 莲(Nelumbo nucifera Gaertner)花色是决定其观赏价值的重要农艺性状之一，但其分子遗传机制尚不清楚。本研究以莲‘满天星’(红花)为母本，‘巨无霸’(白花)为父本构建F₂分离群体。利用简化基因组测序技术(SLAF-seq)构建高密度遗传图谱，根据F₂群体连续两年的表型数据，对莲的红花、白花性状进行QTL分析，开发与该性状紧密连锁的PARMS(Penta-primer amplification refractory mutation system，五引物扩增受阻突变体系)分子标记。结果显示：莲的遗传图谱全长为1046.82 cM，包含8个连锁群、共6376个上图标记，两个相邻标记间的平均图距为0.16 cM。在第6连锁群上检测出1个稳定控制莲红花、白花性状的QTL，贡献率为49.957%，于该定位区域预测到18个基因，其中5个为控制莲红花、白花性状的候选基因。同时，开发了3个与莲红花、白花性状紧密连锁的PARMS标记，能有效区分F₂群体中的红花和白花表型。
- 莲 /
- 花色 /
- QTL定位 /
- SNP分子标记
Abstract: As one of the key agronomic traits that determine ornamental value, the genetic mechanism of flower color in lotus (Nelumbo nucifera Gaertner) requires further exploration. In this study, an F₂ segregating population was obtained with the lotus cultivar ‘Mantianxing’ (red flower) as the female parent and ‘Juwuba’ (white flower) as the male parent. A high-density genetic map of the lotus was constructed using specific locus amplified fragment sequencing (SLAF-Seq). Combined with the phenotypic information of the F₂ individuals obtained over two consecutive years, QTL mapping of the flower color trait was carried out. PARMS molecular markers linked to this trait were also developed. The integrated map spanned a genetic distance of 1046.82 cM, which consisted of 6376 SLAF markers on eight linkage groups, with an average genetic distance of 0.16 cM between adjacent markers. A QTL potentially responsible for red and white flower colors was stably detected on the sixth linkage group, with a contribution rate of 49.957%. Eighteen genes were located in the QTL interval, five of which were identified as candidate genes associated with flower color. Three PARMS markers were developed that could be effectively used for genotyping red and white flowers in F₂ individuals.
- Lotus /
- Flower color /
- QTL mapping /
- SNP markers

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