山金柑实生后代四倍体发掘及形态和代谢评价

张成磊; 周锐; 谢善鹏; 解凯东; 夏强明; 伍小萌; 郭文武

doi:10.11913/PSJ.2095-0837.2022.10047

山金柑实生后代四倍体发掘及形态和代谢评价

1. 台州科技职业学院, 台州市园艺生物技术重点实验室, 浙江台州 318020;
2. 华中农业大学园艺植物生物学教育部重点实验室, 武汉 430070

基金项目:

国家自然科学基金（32172525）；广东省科技计划（2018B020202009）；湖北省科技计划（2020BBA036，2021DFE016）。

详细信息

作者简介:
张成磊(1987-),男,硕士,实验师,研究方向为柑橘种质资源发掘与利用(E-mail:zcl_mail@yeah.net)。

通讯作者:
郭文武,guoww@mail.hzau.edu.cn

中图分类号: S666.1
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出版历程
- 收稿日期: 2021-09-12
- 修回日期: 2021-11-09
- 网络出版日期: 2022-10-31
- 发布日期: 2022-02-27

Exploration and evaluation of morphological traits and primary metabolites of tetraploid seedlings from Hongkong kumquat (Fortunella hindsii Swingle)

1. Taizhou Vocational College of Science & Technology, Taizhou Key Laboratory of Horticultural Biotechnology, Taizhou, Zhejiang 318020, China;
2. Key Laboratory of Horticultural Plant Biology of Ministry of Education/College of Horticulture & Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, China

Funds:

This work was supported by grants from the National Natural Science Foundation of China (32172525),Science and Technology Innovation Strategy Special Fund of Guangdong Province (2018B020202009), and Key Research and Development Program of Hubei Province (2020BBA036, 2021DFE016).

摘要

摘要: 以多胚系山金柑（Fortunella hindsii Swingle）为材料，采用“观根辨叶看油胞”形态初选法，从1289株实生后代筛选出疑似四倍体（双二倍体）8株，流式细胞仪检测和SSR分子鉴定表明它们均为同源四倍体，初选准确率100%，群体自然发生率0.62%。对其形态和初生代谢物进行检测，结果显示：山金柑四倍体株高、茎粗、节间数、节间长、气孔密度均显著低于二倍体，而叶片厚度、气孔大小显著高于二倍体；GC-MS分析鉴定到24种初生代谢物，四倍体叶片奎宁酸含量显著高于二倍体，肌醇、4-氨基丁酸和1-棕榈酸单甘油酯含量显著低于二倍体。
- 山金柑 /
- 多倍体 /
- 流式细胞仪 /
- SSR分子标记 /
- 初生代谢
Abstract: To explore tetraploid seedlings from polyembryonic Hongkong kumquat (Fortunella hindsii Swingle), we screened eight putative tetraploids (doubled diploids) from 1289 seedlings based on root, leaf, and oil gland morphology. Flow cytometry and molecular identification showed that these tetraploids were all autotetraploids, with a screening accuracy of 100% and natural occurrence rate of 0.62%. Compared with diploids, the tetraploids of Hongkong kumquat showed significantly lower plant height, stem diameter, internode number, internode length, and stomatal density, but higher leaf thickness and stomatal size. In addition, leaf primary metabolites between the tetraploids and corresponding diploids were analyzed by gas chromatography-mass spectrometry (GC-MS). Among the 24 detected primary metabolites, the content of quininic acid in the tetraploid leaves was significantly higher than that in the diploid leaves, while the contents of inositol, 4-aminobutyric acid, and 1-monopalmitin were significantly lower.
- Fortunella hindsii /
- Polyploidy /
- Flow cytometry /
- Simple sequence repeat /
- Primary metabolites

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