Identification and formation of natural hybrids of Campeiostachys in Triticeae
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摘要: 本研究通过形态学、细胞学、系统发育分析等方法对小麦族曲穗草属(Campeiostachys)的两株疑似天然杂种进行鉴定,探究其可能的亲本及杂种形成机制。结果显示:两株天然杂种在形态上与短芒曲穗草(Campeiostachys breviaristata(Keng) Y.H.Zhou,H.Q.Zhang et C.R.Yang.)相近,长势优于后者,但其花粉育性(13.30%和21.20%)和结实率(10.70%和18.60%)较低;细胞学分析表明两株天然杂种均为六倍体(2n =42),染色体组组成为StStYYHH;系统发育分析结果表明两株杂种的可能亲本为短芒曲穗草、无芒披碱草(Elymus submuticus Keng)、黑紫披碱草(Elymus atratus (Nevski) Hand.-Mazz)。基于杂种具有无芒或短芒的特征,结合形态学特征,推测短芒曲穗草和无芒披碱草为两株天然杂种可能的亲本供体,且短芒曲穗草为母本。Abstract: In this study, morphological, cytological, and phylogenetic analyses were used to identify two suspected natural hybrids in the genus Campeiostachys. Results showed that the two natural hybrids were similar to C.breviaristata (Keng) Y. H. Zhou, H. Q. Zhang et C. R. Yang. morphologically, but had low pollen fertility (13.30% and 21.20%) and seed setting (10.70% and 18.60%). Cytological studies showed that the two hybrids were hexaploids (2n=42), with StStYYHH genomes. Phylogenetic analysis based on the nuclear gene DMC1 and chloroplast gene rps16 showed that the possible parents of the natural hybrids were C. breviaristata,Elymus submuticus Keng, and Elymus atratus (Nevski) Hand.-Mazz, with C. breviaristata being the female parent. Considering the hybrids had no or short awns, we inferred that C. breviaristata (female parent) and E. submuticus may be the parental species of these two natural hybrids.
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Keywords:
- Campeiostachys /
- Natural hybrids /
- DMC1 /
- rps16
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[1] Arnold ML. Iris nelsonii (Iridaceae):Origin and genetic composition of a homoploid hybrid species[J]. Am J Bot, 1993, 5:577.
[2] Mason-Gamer, Roberta J. Reticulate evolution, introgression, and intertribal gene capture in an allohexaploid grass[J]. Syst Biol, 2004, 53(1):25-37.
[3] 卢宝荣. 小麦族遗传资源的多样性及其保护[J]. 生物多样性, 1995, 3(2):63-68. Lu BR. Diversity and conservation of the Triticeae genetic resources[J]. Chinese Biodiversity, 1995, 3(2):63-68.
[4] Stebbins GL, Vaarama A. Artificial and natural hybrids in the Gramineae, tribe Hordeae Ⅰ. Elymus, Sitanion, and Agropyron[J]. Am J Bot, 1946, 33:338-351.
[5] Stebbins GL, Valencia JI, Valencia RM. Artificial and natural hybrids in the Gramineae, tribe Hordeae. Ⅱ. Agropyron, Elymus and Hordeum[J]. Am J Bot, 1946, 33:579-586.
[6] Stebbins GL, Walters MS. Artificial and natural hybrids in the Gramineae, tribe Hordeae. Ⅲ. Hybrids involving Elymus condensatus and E. triticoides[J]. Am J Bot, 1949, 36:291-301.
[7] Stebbins GL, Pun FT. Artificial and natural hybrids in the Gramineae, tribe Hordeae. Ⅵ. Chromosome pairing in Secale cereale×Agropyron intermedium and the problem of genome homologies in the Triticeae[J]. Genetics, 1953, 38:600-608.
[8] Stebbins GL, Snyder LA. Artificial and natural hybrids in the Gramineae, tribe Hordeae. Ⅸ. Hybrids between western and eastern North American species[J]. Am J Bot, 1956, 43:305-312.
[9] Jensen KB, Redinbaugh MG, Blood M, Horton WH, Asay KH. Natural hybrids of Elymus elymoides×Leymus salinus subsp. salmonis (Poaceae:Triticeae)[J]. Crop Sci, 1999, 39(4):976-982.
[10] 路兴旺, 刘博, 刘瑞娟, 窦全文. 青海高原披碱草属种间天然杂种的细胞学鉴定[J]. 植物研究, 2019, 39(6):846852. Lu XW, Liu B, Liu RJ, Dou QW. Cytogenetic identification on interspecific hybrids in genus Elymus L. of Qinghai Plateau[J]. Bulletin of Botanical Research, 2019, 39(6):846-852. [11] 颜济, 杨俊良. 小麦族生物系统学:第5卷[M]. 北京:中国农业出版社, 2011. [12] 德英, 乌吉玛, 穆怀彬, 解继红, 赵来喜. 3种披碱草属(Elymus)牧草交配系统研究[J]. 植物遗传资源学报, 2015, 16(4):903-906. De Y, Wu JM, Mu HB, Xie JH, Zhao LX. Study on the mating system of three species of Elymus forages[J]. Journal of Plant Genetic Resources, 2015, 16(4):903906. [13] Zhang HQ, Yang RW, Dou QW, Tsujimoto H, Zhou YH. Genomic constitutions of Hystrix patula, H. duthiei ssp. duthiei and H. duthiei ssp. longearistata (Poaceae:Triticeae) revealed by meiotic pairing behavior and genomic in-situ hybridization[J]. Chromosome Res, 2006, 14(6):595-604.
[14] 王龙. 偃麦草属高倍性物种染色体组组成及系统发育研究[D]. 成都:四川农业大学, 2017. [15] Petersen G, Seberg O. Molecular evolution and phylogenetic application of DMC1[J]. Mol Phylogenet Evol, 2002, 22(1):43-50.
[16] Peterson PM, Romaschenko K, Johnson G. A classification of the Chloridoideae (Poaceae) based on multi-gene phylogenetic trees[J]. Mol Phylogenet Evol, 2010, 55(2):580-598.
[17] Lei YX, Liu J, Fan X, Sha LN, Wang Y. Phylogeny and molecular evolution of the DMC1 gene in the polyploid genus Roegneria and its affinitive genera (Poaceae:Triticeae)[J]. Bot J Linn Soc, 2017, 186:129-142.
[18] 陈丽丽, 吴婍, 张昌兵, 李达旭, 白史且. 披碱草属天然杂种形态学鉴定[J]. 草学, 2019(5):24-30. [19] 相吉山. 青藏高原地区小麦族野生近缘植物天然杂种的遗传鉴定[D]. 西宁:青海大学, 2008. -
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