Effects of lanthanum on growth and polyphyllin content of Paris polyphylla Smith var. chinensis (Franch.)Hara
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摘要: 本研究以4年生华重楼（Paris polyphylla Smith var. chinensis（Franch.）Hara）为实验材料，分别采用0、5、20、80、160 mg/L的加镧营养液培养华重楼植株，通过比较不同营养生长指标及采用HPLC法测定根茎皂苷组分，研究不同施镧水平对华重楼生长及皂苷含量的影响。结果显示，镧对华重楼生长及皂苷含量均有显著影响，随着镧溶液浓度的增加，华重楼皂苷含量呈上升趋势，但当镧含量达到160.00 mg/L时，则会对华重楼的生长产生抑制作用，导致株高降低、比叶面积升高。研究结果表明80.00 mg/L的施镧水平最有利于华重楼的营养生长及根茎皂苷积累。Abstract: Four-year-old Paris polyphylla Smith var. chinensis seedlings were cultured in five different concentrations (0, 5, 20, 80 and 160 mg/L) of lanthanum (La) solution. The effects of different application levels of La on the growth and rhizome saponin content were studied by comparing different vegetative growth indexes and components of rhizome saponins of P. polyphylla var. chinensis, as determined by HPLC. Results showed that La significantly impacted the growth and polyphyllin content of P. polyphylla var. chinensis. Polyphyllin content showed an increasing trend with the increase in La concentration; however, La concentrations of 160 mg/L inhibited P. polyphylla var. chinensis growth, which reduced plant height and increased specific leaf area. Overall, this study suggests that a La concentration of 80 mg/L is most conducive to the growth and accumulation of saponin in P. polyphylla var. chinensis.
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