Expression of Human Interferon α-2b Gene in Tobacco(Nicotiana tabacum L.)

Polymerase Chain Reaction(PCR)amplifies the human interferon α-2b(HuIFN α-2b) gene encoding region from the plasmid pAIFN.The encoding region sequence was liga-sed into the pBI121 vector to construct plant eukaryotic expression vector pBIFN.The freeze-thaw method was used to transfect the vector pBIFN into Agrobacterium tumefaciens LBA4404 and then the leaf disc dip method was used to transform the A.tumefaciens LBA4404 embo-died with pBIFN into the tobacco(Nicotiana tabacum L.)leaf explants and plants were regene-rated from cultured transformed tobacco-leaf explants.The molecular analysis methods of PCR,RT-PCR,Western blot and the WISH/VSV were applied to confirm the regenerated tobacco plants and the results showed that the HuIFN α-2b gene was successfully integrated into the tobacco nuclear genome and HuIFN α-2b protein was expressed with activity.This paper documents the expression of the HuIFN α-2b protein in tobacco nuclear transformation system and implies that the potential application of HuIFN α-2b gene in tobacco chloroplast transformation system is feasible.