Guanidine Thiocyanate Method of Total RNA Isolation in Platanus aceriflia

Guanidine Thiocyanate method was used to isolate total RNA from the inflorescence tissue and leaves of Platanus aceriflia successfully with regard to high quantity of phenolic compounds in its tissue.The high-quality total RNA was obtained by guanidine thiocyanate that eliminated the interferences of polyphenols and acquired the 28S rRNA,which is approximately two times as light as the 18S rRNA.The isolated RNA was of sufficient quality for cloning partial cDNA of Leafy gene homologous to Leafy in Arabidopsis thaliana according to RT-PCR.The method described here was the basis of Northern blotting and cloning genes from P.aceriflia according to homologous sequence.