Study on Storage Protein of High Quality Hybrid Rice in Hubei Province

Twenty cultivars of hybrid rice were chosen in this study,of which including ten cultivars of high quality hybrid rice(‘Ezao 17’,‘Ezao 18’,‘Liangyou 1193’,‘Wuxiang 880’,‘Yueyou 26’,‘Yiyou 99’,‘Xieyou 96’,‘Ewan 12’,‘Ezhong 5’,‘Liangyou 277’) identified by Hubei Province in two years and seven ones of general hybrid rice(‘G98-202’,‘578’,‘Liangyou 637’,‘Yiyou 22’,‘3685’,‘3089’,‘JY 948’),and three kinds of high quality hybrid rice which have passed the national identification(’Jiayu 948’,’Liangyou 932’,’Zhou 903’) as control.Using discontinuous SDS-PAGE,the storage glutelin of rice seeds were separated into a 57 kD precursor,a 37-39 kD acidic subunit and a 22-23 kD basic subunit.Five kinds of hybrid rice ‘3089’,‘Jiayu 948’,‘537’,‘Zhou 903’ and ‘Liangyou 932’ were chosen to analyze the content of the glutelin subunit by HPLC(high performance liquid chromatography).The results showed that the content of the glutelin subunit could be regarded as a standard of distinguishing between high and low quality rice.