Establishment and Assessment of Taxus media Cell Line

Taxol in Taxus cell is little and unstable,which prevents the industrialization process of(pro-)(ducing taxol) by cell culture.Using Taxus media as material,the T. media callus was accrued.After subculturing on solid modified B5 medium for 15 time,and supplied with VC,PVP and activated carbon,cell line with the ability of high taxol production was attained.Cell growth cycle,taxol content and cell death rate of T. media(MC),T. cuspidate(NC) which was successfully established at the same time and T. chinensis(SC) which was subcultured for several years in the lab was studied.The results show that the biomass of SC reaches the peak of 5.9 times,which is higher than that of NC and MC.With the same initial inoculation weight,taxol production of MC reached at 9.5 mg/L,and that could reach at 41mg/L after induced by MJ.