Functional Deletion Analysis of FaChit1 Promoter Region from Festuca arundinacea

Activation of different promoter fragments by fungal elicitors,dehydration,mechanical wounding,and ethylene were analyzed in transgenic tobacco using transcriptional fusions of FaChit1 5' upstream sequences to the GUS reporter gene (promoter-GUS expression vectors were designated as pFaChit1P-Ⅰ,pFaChit1P-Ⅱ and pFaChit1P-Ⅲ,respectively).Analysis of promoter deletion showed that the FaChit1 promoter conferred the strongest induction of GUS activity in response to treatment with fungal elicitors,but the least induction in response to mechanical wounding;GUS activity could be induced in response to four stress treatments in leaves containing the 651 bp upstream of the FaChit1 start codon,and the fragment between 935 bp and 233 bp upstream of the FaChit1 start codon was sufficient to direct gene expression in response to fungal elicitors,ethylene,dehydration,and mechanical wounding.Results indicated that the FaChit1 promoter was a multiple stress inducible promoter.