Cloning of Pathogen-Related Protein Gene(SfPR-1) from Salsola ferganica and Its Expression Analysis under Salt Stress

A cDNA fragment was isolated from Salsola ferganica by suppression subtractive hybridization and its full-length cDNA with 817 bp was cloned by SMART^TM RACE, and was named SfPR-1 gene (GenBank accession number: JQ670917). The SfPR-1 gene consisted of a 501 bp open reading frame encoding 166 amino acids with molecular weight of 18.01 kDa and an isoelectric point of 9.37, a 65 bp 5'-UTR and 251 bp 3'-UTR. The deduced amino acid sequence of SfPR-1 showed high identity of 73.6%, 57.8%, 55.5% and 53.9% to those of pathogen-related protein 1 from Beta vulgaris, Arabidopsis thaliana, Nicotiana tabacum and Zea mays, respectively, and had a conserved six-cysteine motif. Reverse transcriptase PCR and Real-time PCR methods were used to investigate the expression profile of the SfPR-1 gene under salt stress. SfPR-1 showed up-regulated expression patterns under salt treatment. Based on our results, we concluded that the SfPR-1 gene might be involved in salt response and an important component for the salt tolerant pathway in Salsola ferganica.