Overexpression of CaNPR1 Enhances Resistance to Ralstonia solanacearum Infection in Tobacco
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Abstract
The NPR1 (nonexpressor of pathogenesis-related genes 1) gene plays an important role in SA-depended defense response processes in model plants such as Arabidopsis and rice.However,the function and underlying mechanism of this gene in non-model plants,including pepper (Capsicum annuum) and other Solanaceae,remain poorly understood.Here,we report on the functional characterization of CaNPR1 from pepper.Our data showed that the full-length cDNA of CaNPR1 was isolated and overexpression of CaNPR1 was construed in tobacco K326.Compared to wild type K326,CaNPR1-OE lines did not exhibit any phenotypic differences,but exhibited higher resistance to Ralstonia solanacearum inoculation.In addition,this was coupled with enhanced transcript levels of defense-related marker genes,suggesting that CaNPR1 possessed higher conservative function in cross species.
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