Establishment for Culture In Vitro and Plant Regeneration System of Citrus grandis cv.Chandler

The cotyledons,cotyledonary nodes,epicotyl segments,and stem segments with lateral buds of aseptic Citrus grandis Osbeck cv.Chandler seedings were used as the explants to study the process of tissue culture rapid reproduction.Results showed that the cotyledonary nodes were the best explants of bush-bud induction(induction rate 100%).The best medium for inducing bush-bud was MS+6-BA 2.0 mg/L+NAA 0.05 mg/L+sucrose 30 g/L+activated carbon 0.4 g/L,the coefficient of proliferation reached 11.2.The optimum medium for shoot rooting was 1/2MS+NAA 0.5 mg/L,which resulted in a rooting rate of 100%.Fifteen days after transplanting,100% of the plantlets survived.