Molecular characteristics and expression pattern analysis of glutathione peroxidase genes in Phyllostachys edulis (Carriere) J. Houzeau

The molecular characteristics and expression patterns of glutathione peroxidase genes (GPX) in moso bamboo (Phyllostachys edulis (Carriere) J. Houzeau) were analyzed using bioinformatics and molecular biology. A total of nine GPX genes were identified from the genome of moso bamboo (PeGPX1-PeGPX9), which all contained six exons and five introns. The lengths of proteins encoded by PeGPXs ranged from 168 to 235 aa, with relative molecular weights of 18.41 to 25.54 kD and isoelectric points of 5.88 to 9.48. Subcellular localization analysis predicted that all PeGPXs were localized on the chloroplast, except for PeGPX5 on mitochondria. A variety of stress and hormone-related cis-acting elements were found in the promoters of PeGPXs. Quantitative real-time polymerase chain reaction (qRT-PCR) showed that the expression levels of PeGPXs in the leaves of moso bamboo were distinctly affected by high light, low temperature, GA₃, NAA, and MeJA treatments.