Cloning and stress expression analysis of the TaHDA19 gene in Triticum aestivum

A RPD3/HDA1 histone deacetylase gene TaHDA19 was amplified from the Triticum aestivum variety ‘Kenong 199’ by gene cloning techniques and analyzed by bioinformatics. Results showed that gene's open reading frame (ORF) was 1560 bp in length and encoded 519 amino acids. The amino acid sequence contained the typical domain Hist-deacety1. Promoter analysis showed that the gene contained a variety of response elements, such as light response elements I-box and G-box, hormone response element ABRE, and low temperature response element LTR. The gene expression pattern of ‘Kenong 199’ in different tissues and under different stress conditions was analyzed. Results showed that the gene was expressed in roots, stems, leaves, and young spikes, with the highest expression found in leaves. In addition, the gene expression levels were different under ABA, NaCl, and PEG treatment for 0, 1, 3, 6, 12, and 24 h. The gene was up-regulated in the 12 growth stages after heat-stress treatment at 35℃ and 42℃ for 1 h compared with the control.