Optimal Total Flavonoids Extraction and Identification of Volatile Oil Components in Miscanthus sinensis

An ultrasonic assisted method was used to study the optimum extraction of flavonoids in Miscanthus. The determined optimal extraction conditions of flavonoids were as follows:70% ethanol concentration, 1:30 ratio of raw material to liquid, 70℃ extraction temperature, 40 min extraction time, and three extract series, respectively. Under these conditions, total flavonoid content of M.lutarioriparius was 7.24 mg/g. The experimental yields of flavonoids from various kinds of M.sinensis were significantly different, ranging from 4.16 mg/g to 7.84 mg/g. The volatile oil components in Miscanthus x giganteus (hexaploid),‘GMG121’, and M.sinensis hybrid were determined by GC/MS, with 28 different components identified. This study provides useful information on genetic breeding, which has great significance for the development and utilization of M.sinensis.