Isolation and Activity Analysis of Cell Wall Invertase Gene Promoter (EhcwINVP) from Elsholtzia haichowensis Sun

Cell wall invertase gene promoter, EhcwINVP (1727 bp), was amplified from the genomic DNA of Elsholtzia haichowensis Sun by hiTAIL-PCR and genome walking PCR. Bioinformatics analysis showed that the promoter fragment contained multiple cis-acting elements involved in the regulation of hormones including abscisic acid, gibberellin, cytokinin, and tolerance to drought, low temperature, and heavy metals such as copper. The pCAMBIA1301 binary vector was digested with restriction enzymes to remove the 35S promoter that drove GUS expression and replaced with EhcwINVP sequences to generate the construct EhNcwINVP::GUS. Histochemical analyses of transgenic Arabidopsis thaliana L. plants indicated that the EhcwINVP promoter was capable of driving GUS expression, and GUS activity in the leaves and roots of transgenic A. thaliana plants increased by about 1.7 and 1.5 times that of the control under copper stress, respectively.