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李想, 周亚东, 黄钰倩, 李小燕, 刘星. 基于核基因和叶绿体基因的台湾水韭遗传多样性和遗传结构的分析[J]. 植物科学学报, 2017, 35(6): 851-855. DOI: 10.11913/PSJ.2095-0837.2017.60851
引用本文: 李想, 周亚东, 黄钰倩, 李小燕, 刘星. 基于核基因和叶绿体基因的台湾水韭遗传多样性和遗传结构的分析[J]. 植物科学学报, 2017, 35(6): 851-855. DOI: 10.11913/PSJ.2095-0837.2017.60851
Li Xiang, Zhou Ya-Dong, Huang Yu-Qian, Li Xiao-Yan, Liu Xing. Analysis on the genetic diversity and structure of Isoetes taiwanensis based on nuclear and chloroplast DNA sequences[J]. Plant Science Journal, 2017, 35(6): 851-855. DOI: 10.11913/PSJ.2095-0837.2017.60851
Citation: Li Xiang, Zhou Ya-Dong, Huang Yu-Qian, Li Xiao-Yan, Liu Xing. Analysis on the genetic diversity and structure of Isoetes taiwanensis based on nuclear and chloroplast DNA sequences[J]. Plant Science Journal, 2017, 35(6): 851-855. DOI: 10.11913/PSJ.2095-0837.2017.60851

基于核基因和叶绿体基因的台湾水韭遗传多样性和遗传结构的分析

Analysis on the genetic diversity and structure of Isoetes taiwanensis based on nuclear and chloroplast DNA sequences

  • 摘要: 利用核基因和叶绿体基因对台湾水韭(Isoetes taiwanensis Devol)2个居群20个样本的遗传多样性和遗传结构进行了分析,并对台湾水韭的保育提出了建议。核基因的检测结果显示,2个居群共存在18个单倍型,单倍型多样性为0.9842,核苷酸多样性为0.00215,居群间基因流Nm为4.26,居群间分化系数Gst值为0.05543;叶绿体DNA的检测结果显示,单倍型数目为6,单倍型多样性为0.6211,核苷酸多样性为0.00326。其中台北居群的单倍型多样性和核苷酸多样性均比金门居群高。核基因和叶绿体基因的AMOVA分析显示,居群内的遗传差异远高于居群间。台湾水韭的核苷酸多样性相对其他水韭属植物较低,可能与台湾水韭的染色体倍型以及狭域分布有关。居群结构的形成可能和基因流以及奠基者效应有关。宜采用原地和迁地保护的方法对居群进行保护。

     

    Abstract: We employed both nuclear and chloroplast DNA sequences to analyze the genetic diversity and structure of 20 individuals from two separate populations of Isoetes taiwanensis, with some suggestions for the conservation of this species also proposed. In the nuclear DNA data set, 18 haplotypes were discovered, with a haplotype diversity of 0.9842 and nucleotide diversity of 0.00215. In the chloroplast DNA data set, six haplotypes were detected, with a haplotype diversity of 0.6211 and nucleotide diversity of 0.00326. AMOVA results indicated that genetic variations within populations were larger than those among populations. Based on nucleotide DNA sequences, gene flow was 4.26 and Gst was 0.05543. Compared with other Isoetes species, the nucleotide diversity of I. taiwanensis was relatively low, possibly related to the ploidy level and narrow distribution. The genetic structure is likely related to strong gene flow and foundation effects. Both in situ and ex situ conservation is recommended.

     

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