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张柯彬, 陈炳全, 刘志雄. 甜荞FaesAP2基因的克隆与表达分析[J]. 植物科学学报, 2017, 35(3): 354-361. DOI: 10.11913/PSJ.2095-0837.2017.30354
引用本文: 张柯彬, 陈炳全, 刘志雄. 甜荞FaesAP2基因的克隆与表达分析[J]. 植物科学学报, 2017, 35(3): 354-361. DOI: 10.11913/PSJ.2095-0837.2017.30354
Zhang Ke-Bin, Chen Bing-Quan, Liu Zhi-Xiong. Cloning and expression analysis of the FaesAP2 gene from Fagopyrum esculentum(Polygonaceae)[J]. Plant Science Journal, 2017, 35(3): 354-361. DOI: 10.11913/PSJ.2095-0837.2017.30354
Citation: Zhang Ke-Bin, Chen Bing-Quan, Liu Zhi-Xiong. Cloning and expression analysis of the FaesAP2 gene from Fagopyrum esculentum(Polygonaceae)[J]. Plant Science Journal, 2017, 35(3): 354-361. DOI: 10.11913/PSJ.2095-0837.2017.30354

甜荞FaesAP2基因的克隆与表达分析

Cloning and expression analysis of the FaesAP2 gene from Fagopyrum esculentum(Polygonaceae)

  • 摘要: 本研究采用同源克隆和RACE技术,从甜荞(Fagopyrum esculentum Moench.)品种'西农9976'中分离出调控花器官发育的FaesAP2基因,该基因序列全长1668 bp,包含1个长为1374 bp的完整开放阅读框,共编码457个氨基酸。序列比对以及系统发育分析结果发现,FaesAP2蛋白拥有2个高度保守的AP2(APETALA2)结构域,在第1个AP2结构域前端有1段由10个氨基酸残基组成的高度保守的核定位信号区;系统发育分析显示其与拟南芥(Arabidopsis thaliana(L.) Heynh.) AP2转录因子的亲缘关系较近。基因表达模式分析表明,该基因在甜荞pin型和thrum型花的雄蕊和雌蕊中有明显的表达,但在幼叶和花被片中不表达,且其表达量在2种类型花不同发育时期呈现明显的变化,均在花药迅速膨大期达到最高值,因此推测该基因在甜荞花发育过程中可能参与了花器官发育的调控。

     

    Abstract: The FaesAG gene,which regulates floral development,was isolated from common buckwheat (Fagopyrum esculentum Moench.) ‘xinong9976’ using homologous cloning and RACE methods.Results showed that the FaesAG cDNA was 1668 bp long with a 132 bp 5'leader region,162 bp 3'untranslated region with a poly-A tail,and 1374 bp ORF encoding 457 amino acids.Sequence alignment and conceptual translation revealed that FaesAP2 contained two highly conserved AP2 domains and a putative nuclear localization signal in front of the first AP2 domain.Phylogenetic analyses demonstrated that FaesAP2 is close to the AP2(APETALA2) transcript factor from Arabidopsis thaliana(L.) Heynh.Expression analysis showed that FaesAP2 was mainly expressed in the developing stamen and gynoecium of the pin and thrum flowers,respectively,but was absent from the developing leaves and tepals.Moreover,FaesAP2 expression persisted throughout all stages of pin and thrum flower bud differentiation.When the anthers rapidly increased in size,FaesAP2 expression reached the highest level in the pin and thrum flowers.These results show that FaesAP2 might be involved in regulating floral organogenesis during Fagopyrum esculentum flower development.

     

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