银杏叶愈伤组织和叶中超氧化物歧化酶、总黄酮类抗氧化活性剂活性的比较分析

Comparative Assay of Superoxide Dismutase and Total Flavone Antioxidant Activities in Leaf-Callus and Leaves of Ginkgo biloba L.

摘要: 以银杏(Ginkgo biloba L.)叶片为外植体进行愈伤组织的诱导和培养,筛选到适合银杏叶片愈伤组织诱导和继代的培养基。以此愈伤组织为研究材料,比较了叶片愈伤组织和叶片中两种活性成分超氧化物歧化酶(SOD)、黄酮的活性差异。结果表明,银杏叶片愈伤组织中SOD的活性明显比叶片中的SOD活性高,同时愈伤组织中SOD同工酶谱带也比叶中有更多表达,但愈伤组织中总黄酮含量却比叶片中的含量低。实验证实SOD和黄酮类物质具有清除超氧阴离子的作用,表明银杏叶片愈伤组织和叶片中具有不同的占主导作用的抗氧化活性的机制。

Abstract: Optimum media for Ginkgo biloba L.leaf callus induction and successive transfer culture were investigated.Callus that arose from Ginkgo leaf explants was used as the research materials,and two varieties of active integrants,superoxide dismutase(SOD) and total flavones,were compared between leaves and its callus.The results demonstrated that SOD activity in callus was markedly higher than that in leaves,as were SOD isoenzymes.However,the content of flavones in callus was lower than that in leaves.The fact that SODs and flavones can scavenge the production of superoxide anion indicated that different predominant mechanisms were used to resist free radicals in Ginkgo callus and leaves.