亚热带一些常见木本油料树种鲜叶提取物清除自由基的活性初探

胡丰林; 陆瑞利; 何云核; 许成林

亚热带一些常见木本油料树种鲜叶提取物清除自由基的活性初探

Studies on Free Radical Scavenging Activities of Extracts from Leaves of Some Subtropical Oil Trees

摘要

摘要: 用二苯基苦基苯肼自由基酶标仪法,对亚热带常见的90余种油料树种鲜叶80%甲醇提取物的自由基清除活性进行了比较,发现所有树种提取物的自由基清除率都随浓度的增加和随着37℃下孵育时间的延长而增大。其中,苦茶槭、金缕梅、黄连木、茶、三角枫、黄山栾树、山麻杆、红瑞木、木、秀丽槭、算盘子、木蜡树、油茶、米心水青冈、乌桕、栾树和山茶等树叶提取物在相当于鲜叶浓度0.5mg/mL时,37℃下孵育20min后的自由基清除率分别达96.7%、93.2%、90.6%、87.5%、86.4%、85.0%、84.3%、82.1%、80.8%、77.1%、74.5%、72.5%、72.3%、68.7%、63.8%、62.0%和61.7%。这些树种有较大的开发潜力。

Abstract: A DPPH-Microplate assay were made to determine the free radical scavenging activity of the 80% methanol extracts from the leaves of subtropical oil trees. The results revealed that all trees have different free radical scavenging capacities, and as the raising of concentration and the prolonging of incubation time at 37℃, their free radical scavenging capacities increased. Of all trees, Acer ginnala Maxim. ssp. theiferum (Fang) Fang, Hamamelis mollis Oliv., Pistasia chinensis Bunge, Camellia sinensis (L.) O.Ktze., A. buergerianum Miq.,Koelreuteria bipinnata Franch. var. integrifiola (Morr.) T.,Alchornea davidii Franch.,Cornus alba L.,Loropetalum chinense R.Br.,Acer elegantulum Fang et Chiu,Glochidion puberum (L.) Hutch.,Toxicodendron sylvestre O.Kuntze,Camellia oleifera Abel.,Fagus engleriana Seem., Sapium sebiferum (L.) Roxb., Koelreuteria paniculata Laxm. and Camellia japonica L. have the strongest free radical scavenging capacity. The extracts at a concentration that equals to 0.5 mg leaves per milliliter, after incubated for 20 minutes they can reach a DPPH decrease rate of 96.7%, 93.2%, 90.65%, 87.5%, 86.4%, 85.0%, 84.3%, 82.1%, 80.8%, 77.1%, 74.5%, 72.5%, 72.3%, 68.7%, 63.8%, 62.0% and 61.7% respectively.

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