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刘明志. 大叶紫花苜蓿愈伤组织原生质体再生植株[J]. 植物科学学报, 1996, 14(4): 329-333.
引用本文: 刘明志. 大叶紫花苜蓿愈伤组织原生质体再生植株[J]. 植物科学学报, 1996, 14(4): 329-333.
Liu Mingzhi. PLANT REGENERATION FROM CALLUS PROTOPLASTS OF MEDICAGO SATIVA SSP. SATIVA[J]. Plant Science Journal, 1996, 14(4): 329-333.
Citation: Liu Mingzhi. PLANT REGENERATION FROM CALLUS PROTOPLASTS OF MEDICAGO SATIVA SSP. SATIVA[J]. Plant Science Journal, 1996, 14(4): 329-333.

大叶紫花苜蓿愈伤组织原生质体再生植株

PLANT REGENERATION FROM CALLUS PROTOPLASTS OF MEDICAGO SATIVA SSP. SATIVA

  • 摘要: 大叶紫花苜蓿下胚轴诱导的愈伤组织在继代培养基上生长快速, 易于分散。继代第12d的愈伤组织原生质体的得率为6.5×107/g鲜重。原生质体培养基为SH基本培养基, 含有1.0mg/L2, 4-0、0.5mg/LBA、2.0g/LCH、2%蔗糖、6%葡萄糖、5mmol/LMES, 培养密度为1.0×105/mL.培养至第12d时的原生质体再生细胞植板率为3.7%.由原生质体形成的小愈伤组织在含2.0mg/L2, 4-D的MS固体培养基上大量增殖。增殖的愈伤组织转移至2.0mg/L2-ip+0.1mg/LNAA的B5培养基上, 形成体细胞胚并发育成完整植株。

     

    Abstract: Calli derived from hypocotyledon of Medicago sativa ssp. sativa grew rapidly and dispersed easily on subculture media, Protoplasts isolated from calli of l2 days after subculture at a high yield of 6.5×l07/g fresh weigh. Protoplasts were cultured on modified SHmedium containing l.0mgl/L 2, 4-D, 0.5mg/L BA, 2.0g/L CH, 2%sucrose, 6% glucose, 5 mmol/L 2-(N-morpholino)-ethanesulfonic acid with plating density of 1.0×105/mL. Protoplast plating efficiency was 3.7%after 12 days in culture. Microcalli derived from protoplasts multiplicated on solid MSmedium containing 2.0mg/L 2, 4-D. Somatic embryos and plant regeneration were obtained when these calli were transferred to B5 medium containing 2.0mg/L 2-ip and 0.1mg/L NAA.

     

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