Abstract: The karyotype analysis based on mitotic cells in rice, Oryza sativa L.(2n=24), was conducted. Root tips were treated with KCl and enzyme and stained with Giemsa. Twelve pairs of chromosomes are arranged according to the length. The karyotype of rice analysed in this work is n =5m+6sm+1st and 2 pairs of satellite chromosomes were observed in numerous cells.